Sains Malaysiana 53(2)(2024): 359-367

http://doi.org/10.17576/jsm-2024-5302-10

A New Oxoaporphine and Liriodenine's Anti-Neuroblastoma Potential from the Roots of Polyalthia bullata King

(Sebatian Oksoaporfina Baharu dan Potensi Anti-Neuroblastoma Liriodenin daripada Akar Polyalthia bullata King)

PHOEBE SUSSANA PRIMUS¹, CAROL HSIN-YI WU², CHAI-LIN KAO³ & YEUN-MUN CHOO^1,*

¹Department of Chemistry, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia

²Division of Cellular and Immune Therapy, Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan

³Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Taiwan

Received: 4 July 2023/Accepted: 24 January 2024

Abstract

Polyalthia bullata King’s root yielded a new compound named 5-methylliridine (1) in addition to six previously identified compounds. These known compounds include liriodenine (2), 11-methoxyliriodenine (3), lysicamine (4), onychine (5), 5-hydroxy-6-methoxyonychine (6), and 8-methoxyeupolauridine (7). The structures of compounds 1-7 were determined through spectroscopic analysis. Liriodenine (2) exhibited a remarkable ability to decrease the cell viability of cancerous N2A cells to 22% within a 24 h timeframe, indicating its potential as an anti-neuroblastoma agent. Molecular docking results additionally suggested that oxoaporphines (1-4) have the potential to act as inhibitors of protein kinases. These findings highlight the therapeutic potential of P. bullata constituents in cancer treatment, particularly neuroblastoma, and contribute to understanding its medicinal properties.

Keywords: Anti-neuroblastoma; oxoaporphine; protein kinase; Polyalthia bullataKing; Tongkat Ali Hitam

Abstrak

Akar Polyalthia bullata King menghasilkan satu sebatian baharu yang dikenali sebagai 5-metilliridine (1) bersama dengan enam sebatian yang sebelum ini telah dikenal pasti. Sebatian yang telah dikenali ini termasuk liriodenine (2), 11-metoksiliriodenine (3), lysicamine (4), onychine (5), 5-hidroksi-6-metoksionychine (6) dan 8-metoksieupolauridine (7). Struktur bagi sebatian 1-7 telah ditentukan melalui analisis spektroskopi. Liriodenine (2) menunjukkan keupayaan yang luar biasa dalam mengurangkan sel kanser N2A kepada 22% dalam jangka masa 24 jam, memberikan petanda tentang potensi sebagai agen anti-neuroblastoma. Hasil daripada dok molekul turut mencadangkan bahawa oksoaporfina (1-4) mempunyai potensi untuk bertindak sebagai perencat protein kinase. Penemuan ini menonjolkan potensi terapeutik bahan aktif P. bullata dalam rawatan kanser, khususnya neuroblastoma, serta menyumbang kepada pemahaman tentang sifat perubatannya.

Kata kunci: Anti-neuroblastoma; oksoaporfina; Polyalthia bullataKing; protein kinase; Tongkat Ali Hitam

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*Corresponding author; email: ymchoo@um.edu.my